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Acetylated Low Density Lipoprotein, Human

NO.:L8940
Quantity: 3

Application

Lipid metabolism

Description

Concentration: >2mg/ml protein(Based on actual label concentration).
Purity: 98%, is determined agarose gel electrophoresis.
Specifications: 0.22 micron membrane filtered, aseptically filled. Cell Culture Tested.
Storage:
This product is stable for 6 weeks after receipt when handled aseptically and stored at 2-8°C (Do not freeze). After prolonged storage, some precipitate may be observed. This is normal for this product. Clarify out the aggregates by spinning in centrifuge tube for 2 minutes.
Biological Activity: 
Sample lots of Acetylated LDL are evaluated for receptor binding to peritoneal macrophages in conjunction with our DiI-Ac-LDL  and [I-125] Ac-LDL.
Preparation:
Human LDL (which was purified to homogeneity via ultra-centrifugation (1.019-1.063g/cc)) is acetylated with acetic anhydride and dialyzed. It is ultrafiltered through a membrane and packaged aseptically under nitrogen. Each lot is analyzed on agarose gel electrophoresis for migration versus LDL. This lot of Ox-LDL migrates 1.8fold further than the native LDL.
Special note:
1. Do not freeze.
2. Preparations of Ac-LDL are fairly unstable, prepare your experiments in advance and use fresh        material.
3. For research use only.