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Annexin V-FITC/PI Apoptosis Detection Kit

NO.:CA1020
Quantity: 10

Application

Cell apoptosis detection.

Description

Apoptosis, or programmed cell death, is a mechanism of cells used to negatively select cells that are deleterious to the host. The cellular changes involved in the process include loss of phospholipid asymmetry during the early stages of apoptosis. In living cells, phosphatidylserine is transported to the inside of the lipid bilayer by the Mg-ATP dependent enzyme, aminophospholipid translocase. At the onset of apoptosis, phosphatidylserine, which is normally found on the internal part of the plasma membrane, becomes translocated to the external portion of the membrane. The phosphatidylserine becomes available to bind to the annexin V-FITC conjugate in the presence of calcium. Apoptosis may be either spontaneous or induced by incubating the cells with staurosporine. The cells are incubated with annexin V-FITC and propidium iodide(PI). After a 10 minute incubation period at room temperature the cells are analyzed by flow cytometry. Annexin V-FITC is detected as a green fluorescence and propidium iodide is detected as a red fluorescence.


2.《Compound 331 selectively induces glioma cell death by upregulating miR-494 and downregulating CDC20》
Author:Lei Zhang, Tianhui Niu, Yafei Huang, Haichuan Zhu,
《Scientific Reports 5, Article number: 12003 (2015)》
IF:4.259
PMID:26153143
  • DMEM(H, with Penicillin-Streptomycin)

  • RPMI Medium 1640

  • Trypsin-EDTA solution,0.25% (without phenol red)

  • Cryopreservation Medium

  • Annexin V PE/7-AAD Apoptosis Detection Kit