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MTT Cell Proliferation and Cytotoxicity Assay Kit

NO.:M1020
Quantity: 10

Application

The kit is designed for the spectrophotometric measurement of cell growth as a function of mitochondrial activity in living cells.

Description

The kit is designed for the spectrophotometric measurement of cell growth as a function of mitochondrial activity in living cells. Using MTT could measure the activity of living cells via mitochondrial dehydrogenase activity with simple, accurate and reproducible functions. Dehydrogenases in mitochondria can deoxidize MTT to crystalline purple product Formazan. The crystals can be dissolved in specific solvent. Use Microplate Absorbance Reader to measure absorbance near 490nm wavelength. An increase in cell number results in an increase in the amount of MTT Formazan formed and an increase in absorbance.
For best results, cell numbers should be determined during log growth stage. Each test should include a blank containing complete culture medium without cells.

1.《Metabolomic Analysis of Mouse Embryonic Fibroblast Cells in Response to Autophagy Induced by Acute Starvation》
Author:Sensen Shen, Rui Weng, Linnan Li, Xinyuan Xu, Yu B
《Scientific Reports 6, Article number: 34075 (2016)》
IF:4.259
PMID:27703171
  • Cell Counting Kit-8

  • Penicillin-Streptomycin Solution,100×

  • Hanks (with Ca2+& Mg2+, phenol red)

  • 1M Hepes (pH7.2-7.4×sterile)

  • PBS,1× (pH7.2-7.4,0.01M)

  • DMEM(H, with Penicillin-Streptomycin)